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Determination of ergothioneine in Agaricus bisporus by HPLC
Author(s): ZHOU Nian-bo1, ZHU Yan-qin2, YIN Qin-hong3
Pages: 271-
272,279
Year: 2010
Issue:
10
Journal: Food Science and Technology
Keyword: 高效液相色谱法; 双孢菇; 麦角硫因;
Abstract: 目的:采用高效液相色谱法测定双孢菇中麦角硫因的含量。方法:采用十八烷基硅烷键合硅胶(250mm×4.6mm,5μm)为固定相,以乙腈-水(3∶97,v/v)为流动相,在254nm波长测定麦角硫因。结果:麦角硫因在0.0148~0.37μg范围内线性关系良好(r=0.9999),回收率为97.90%,RSD为1.10%。结论:该方法简便准确,重复性好,可作为双孢蘑菇中麦角硫因含量测定方法。
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