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Expression profiling and binding characterization of dimeric chemosensory protein 6 (HarmCSP6) in Helicoverpa armigera (Hübner) (Lepidoptera:Noctuidae)
Author(s): 
Pages: 615-622
Year: Issue:  6
Journal: Acta Entomologica Sinica

Keyword:  棉铃虫 化学感受蛋白 原核表达 荧光定量PCR 二聚体 结合实验;
Abstract: 为了研究棉铃虫Helicoverpa armigera(Hübner)化学感受蛋白(chemosensory proteins,CSPs)在嗅觉识别过程中的功能,本研究克隆并在原核细胞中表达了棉铃虫化学感受蛋白HarmCSP6,Western blot和快速液相色谱(fast protein liquid chromatography,FPLC)检测证实该HarmCSP6蛋白以二聚体的形式表达。实时荧光定量PCR检测发现HarmCSP6基因在雌雄虫触角中表达量很高,在雌虫足及翅中也有一定程度的表达。该二聚体蛋白与22种气味分子的荧光竞争结合实验结果表明,该蛋白与醛类及萜烯类等气味小分子具有较强的结合能力。通过研究该蛋白与小分子的结合,筛选出合适的气味,从而为开发棉铃虫引诱剂及驱避剂提供理论依据。
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