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Issue:
Reconstruction of If channel in HCN4 transfected mesenchymal stem cells in rats
Author(s):
TONG Min
,
YANG Xiang-jun
,
HAN Lian-hua
,
LI Hong-xia
,
ZHAO Xin
,
ZHOU Ya-feng
,
JIANG Wen-ping
Pages:
749
-
753
Year:
2010
Issue:
7
Journal:
Basic & Clinical Medicine
Keyword:
骨髓间充质干细胞
;
慢病毒
;
超极化激活环核苷酸门挖通道
;
If
;
Abstract:
目的 研究慢病毒载体介导超极化激活的环核苷酸门控通道基因4(HCN4)转染大鼠骨髓间充质干细胞(rMSCs)重建起搏离子通道的可行性. 方法 全骨髓法分离大鼠股骨及胫骨的MSCs,流式细胞术鉴定rMSCs;应用四质粒表达系统构建包含目的 基因HCN4的慢病毒载体lentiV-HCN4-GFP,对照组慢病毒载体为仅含有报告基因GFP的慢病毒载体lentiV-GFP;慢病毒载体转染rMSCs;RT-P...
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