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Optimization of phenol-sulfuric acid method for determination of polysaccharide content in Phellinus vaninii L jub mycelium
Author(s): 
Pages: 389-391
Year: Issue:  5
Journal: Science and Technology of Food Industry

Keyword:  瓦尼木层孔菌 多糖 苯酚硫酸法;
Abstract: 对瓦尼木层孔菌菌丝体多糖含量的测定进行了探讨,研究了在苯酚-硫酸法中吸收波长、显色温度、显色时间、浓硫酸用量、苯酚用量等因素对显色结果的影响。通过实验发现,最佳显色条件是波长492nm、显色温度100℃、显色时间20min、浓硫酸用量4.5mL、苯酚用量0.4mL,平均加样回收率100.7%,相对标准偏差(RSD)为0.67%。
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