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Ultrastructural Changes of Ovine Oocytes Cultured In Vitro
Author(s): 
Pages: 223-227
Year: Issue:  2
Journal: ACTA SCIENTIARUM NATURALIUM UNIVERSITATIS NEIMONGOL

Keyword:  绵羊卵母细胞卵泡大小卵丘细胞层多少超微结构;
Abstract: 实验利用透射电镜研究了根据卵泡大小和卵丘细胞层多少分类后各类绵羊卵母细胞在体外成熟培养前后超微结构的变化,结果表明:小卵泡(直径小于1mm)卵经过体外培养其线粒体、皮层颗粒等细胞器均未达到成熟卵母细胞中这两种超微结构的分布状态;大、中两类卵泡(直径大于3 mm和1~3 mm)卵经过体外培养细胞质成熟较好,但其线粒体的分布与体内成熟卵稍有差异,说明当前的培养系统并不能支持卵胞质充分成熟.从线粒体、皮层颗粒、微绒毛、脂滴等超微结构来看,3)(裸卵)、4)(卵丘细胞已扩散)类卵母细胞为退化的卵母细胞,其中裸卵的退化程度更高.
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