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Somatic Meiosis-like Reduction Induced at High Frequency by Nucleotide Analogues in Vicia faba
Author(s): 
Pages: 147-150,附页
Year: Issue:  2
Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY

Keyword:  蚕豆核酸类似物类减数分裂;
Abstract: 建立了用咖啡因、尿嘧啶处理蚕豆(Vicia faba)根尖细胞,诱导高频率体细胞类减数分裂的体系.筛选出最佳药物处理浓度及最佳恢复时间,咖啡因处理过程中减数分裂的频率最高达54.0 %,尿嘧啶处理类减数分裂频率最高达35.7%.观察体细胞类减数分裂的全过程,发现体细胞染色体减数存在着两种类型, 其一为染色体减数分组,多数情况下染色体分为两个组,但未见纺锤丝的作用;另一类型为与配子发生过程相似的减数分裂,出现染色体配对、交叉的图样.这一体系的建立对单倍体的诱导及细胞工程育种有着重要意义.
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