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A proteomic study of serum peptidome patterns of human systemic lupus erythematosus based on MALDI-TOF mass spectrometry analysis
Author(s): WEN Jin-Li, DAI Yong, HUANG Li-Ling, HU Cheng-Xiao, WANG Lin-Xian
Pages: 265-
268,273
Year: 2011
Issue:
3
Journal: Chinese Journal of Immunology
Keyword: 系统性红斑狼疮; 蛋白质组学; 质谱; 分析;
Abstract: 目的:本实验应用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)寻找SLE患者血清中与疾病相关及条件特异的差异性表达多肽,尝试建立SLE的多肽鉴别诊断模型.方法:将SLE患者分为活动期和非活动期两组,以健康志愿者作为正常对照组.应用弱阳离子交换磁珠分离纯化血清样本,MALDI-TOF-MS获取样本生物学信息,建立分类预测模型.结果:成功检测并鉴别出了一系列的差异性多肽,建立了具有高的预...
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