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Protective effects of aFGF on vascular endothelial cells damaged by cisplatin in vitro
Author(s): 
Pages: 347-349
Year: Issue:  3
Journal: Chinese Pharmacological Bulletin

Keyword:  酸性成纤维细胞生长因子顺铂血管内皮细胞细胞培养鉴定保护;
Abstract: 目的 研究酸性成纤维细胞生长因子(aFGF)对顺铂(DDP)引起的体外培养的血管内皮细胞损害的保护作用.方法 通过组织块法获取血管内皮细胞、Ⅷ因子相关抗原免疫细胞化学染色鉴定,纯化后传代接种于96孔培养板:(1)培养24 h后加入一系列浓度的DDP,再培养24 h后用MTT法检测细胞存活率;(2)培养24 h后在系列DDP浓度的基础上加入一定浓度的aFGF,继续培养24h后用MTT法检测细胞存活率...
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