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The gene expression of pitx3 and apoptosis in the cultured neural stem cells from embryonic rats\' midbrain
Author(s): 
Pages: 22-25
Year: Issue:  22
Journal: Chinese Journal of Practical Nervous Diseases

Keyword:  pitx3凋亡;
Abstract: 目的 探讨胚胎大鼠腹侧中脑源性神经干细胞培养过程中pitx3、TH以及凋亡基因的表达.方法 分离E14.5 d胚胎大鼠腹侧中脑组织,用机械吹打方法形成细胞悬液培养,传代7 d、14 d、28 d后,用real-time PCR检测pitx3、Bax、Bcl-2的表达情况.结果 在培养的神经干细胞克隆中,pitx3、Bax基因7~21 d表达逐渐增加,而Bcl-2 基因表达逐渐下降.结论 胚胎大鼠腹...
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