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The protective effect of EGCG on hydrogen peroxide induced SH-SY5Y cell injury
Author(s): 
Pages: 320-324
Year: Issue:  3
Journal: Chinese Pharmacological Bulletin

Keyword:  baxbcl-2实时荧光定量PCR;
Abstract: 目的 观察表没食子儿茶素没食子酸酯(EGCG)对过氧化氢(H2O2)诱导的SH-SY5Y神经细胞凋亡的保护作用.方法 采用体外细胞培养的方法,建立SH-SY5Y神经细胞H2O2损伤模型.通过观察细胞形态,测定细胞存活率(MTT法)、乳酸脱氢酶(LDH)活性,实时荧光定量(real-time)PCR检测细胞bax、bcl-2 mRNA表达的变化.结果 同H2O2组比较,终浓度为50、100、150、...
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