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Dynamic change of T lymphocytes in neonatal rats with hyperoxic lung injury
Author(s): LIU Jin-sheng, XIAO Zhi-hui
Pages: 227-
230
Year: 2011
Issue:
3
Journal: The Journal of Clinical Pediatrics
Keyword: 肺损伤; 高氧; 免疫; 大鼠;
Abstract: 目的 探讨高氧致新生大鼠肺损伤过程中T淋巴细胞的动态变化.方法 新生12 h内的SD大鼠随机分为对照组、高氧组,对照组置于正常空气中喂养,高氧组持续暴露于80% ~ 85%氧浓度下.两组分别于第1、4、7、14、21天时提取肺脏、脾脏和胸腺,以常规组织学方法观察肺脏形态结构变化;运用常规计数法检测脾脏中淋巴细胞数量变化;采用流式细胞术检测脾脏中CD4+、CD8+ T细胞的百分比变化;以混合淋巴细胞...
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