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Myocardial protection of edaravone against isoprenaline-induced oxidative stress and endoplasmic reticulum stress in H9c2 cells
Pages: 410-415
Year: Issue:  3
Journal: Chinese Pharmacological Bulletin

Keyword:  依达拉奉异丙肾上腺素内质网应激氧化应激线粒体膜电位H9c2心肌细胞;
Abstract: 目的 探讨依达拉奉(EDA)能否保护H9c2心肌细胞对抗异丙肾上腺素(ISO)诱导的氧化应激和内质网应激(ERS).方法 用ISO处理H9c2心肌细胞,建立β1肾上腺素受体持续兴奋诱导心肌细胞毒性的体外模型.EDA在ISO处理心肌细胞前1 h加入培养基中作为预处理.CCK-8比色法检测细胞存活率;双氯荧光素(DCFH-DA)染色/荧光显微镜照相检测细胞内活性氧(ROS)的含量;罗丹明123(Rh1...
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