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Separation and Determination of Four Organic Acids in Picrorhiza Scrophulariiflora Pennell by Electrostacking-Large Volume Sample Stacking-Sweeping-Micellar Electrokinetic Chromatography
Author(s): 
Pages: 208-212
Year: Issue:  2
Journal: Chinese Journal of Analytical Chemistry

Keyword:  胶束电动毛细管色谱 电堆积 大体积进样 扫集法 异阿魏酸 肉桂酸 阿魏酸 香草酸;
Abstract: 建立了以电堆积大体积进样-在线扫集-胶束毛细管电动色谱法测定胡黄连中的异阿魏酸、肉桂酸、阿魏酸和香草酸的新方法。考察了pH值、四硼酸钠浓度、SDS浓度、电压、有机溶剂和进样时间对分离效果的影响。以40 mmol/L四硼酸钠-80 mmol/L十二烷基磺酸钠(SDS)为缓冲液(含10%(V/V)甲醇,pH 9.4),在进样电压-10 kV,分离电压20 kV,检测波长214 nm,环境温度25℃的条件下,达到最佳的分离效果。异阿魏酸、肉桂酸、阿魏酸和香草酸的线性范围分别为16~543μg/L,16~518μg/L,18~589μg/L和14~161μg/L;回收率分别为93%~111%,102%~107%,96%~108%和103%~108%;峰面积的RSD均小于4%;4种有机酸的检出限分别为618,148,368和76 ng/L。
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