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Expression and significance of ENPP1, TNAP and ANK proteins in the degeneration of endplate chondrocytes in rats
Author(s): 
Pages: 189-192
Year: Issue:  3
Journal: National Medical Journal of China

Keyword:  Intervertebral diskCell cultureImmunoblottingPyrophosphatases;
Abstract: 目的 观察大鼠终板软骨细胞体外自然退变过程中焦磷酸环路关键酶(ENPP1、TNAP、ANK蛋白)表达的变化.方法 采用连续酶消化及自然传代法,选取原代至第四代终板软骨细胞,用免疫印迹方法分析各代终板软骨细胞中ENPP1、TNAP、ANK蛋白的表达.结果 ENPP1、TNAP、ANK蛋白在各代终板软骨细胞中均有表达;随着细胞的传代,蛋白表达量逐渐减少,第三、四代的表达微弱,ENPP1、TNAP、AN...
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