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Rapid Analysis of Triterpenoidic Saponins in Anemone Raddeana Using Electrospray Ionization Multi-stage Mass Spectrometry Combined with Silica Gel Column Chromatography
Pages: 219-224
Year: Issue:  2
Journal: Chinese Journal of Analytical Chemistry

Keyword:  全扫描电喷雾多级质谱 两头尖 皂苷;
Abstract: 建立了电喷雾多级质谱结合硅胶柱层析快速、全面分析中药两头尖中三萜皂苷成分的方法。取两头尖粉末30 g,在60℃下以300 mL 70%甲醇溶液提取6次,合并提取液,减压浓缩得提取物3.3 g,提取物悬浮于20 mL水中,先用乙酸乙酯(20 mL×3)萃取除脂,再用正丁醇(20 mL×6)萃取。取正丁醇萃取物1.5 g进行硅胶柱层析,以甲醇-氯仿-水(1∶6∶0.05→1∶4∶0.1→1∶2∶0.2→1∶1∶0.3,V/V)溶剂系统的进行梯度洗脱。通过TLC比较,将两头尖皂苷粗提物分成4个组分。通过全扫描电喷雾多级质谱对每个组分分别进行分析,并结合文献报道,确定了两头尖中16个已知皂苷的结构,对3个未知皂苷的结构进行了推测。
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