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Rapid Desalting of Recombinant Human Erythropoietin Sample by Microelectro Purification Device for Mass Spectrometric Detection
Author(s): 
Pages: 231-234
Year: Issue:  2
Journal: Chinese Journal of Analytical Chemistry

Keyword:  液相色谱 质谱 微电纯化装置 超滤 重组人促红细胞生成素;
Abstract: 利用实验室构建的微电纯化装置(Microelectro purification device,MEPD),采用电喷雾质谱正离子模式检测,在2 min之内实现重组人促红细胞生成素(recombinant human erythropoietin,rhEPO)的在线脱盐,同时和传统的C18色谱柱(150 mm×4.6 mm,5μm)梯度洗脱以及超滤管除盐结果进行比较。利用MEPD除盐后,rhEPO特征离子信噪比增加,检测灵敏度明显提高,操作时间大大缩短。本方法简单、快速,可作为质谱分析rhEPO样品的前处理方法。
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