IMMUNOSCREENING OF cDNA CLONES EXPRESSING SCHISTOSOMA JAPONICUM ANTIGENS BY SERUM FROM REINFECTED PEOPLE
Author(s): WANG Rongzhi, WU Haizuo, CHEN Shuzhen, ZHANG Zhaosong, SU Chuan, WU Guanling
Pages: 67-69
Year: 2000 Issue: 2
Journal: CHINESE JOURNAL OF ZOONOSES
Keyword: Schistosomajaponicum; Reinfected people; Immunoscreening; cDNA clones;
Abstract: To obtain waluable Schistosoma japonicum (Sj) antigen clones, an constructed Sj adult worm cDNA library was screened with serum from reinfected people of Sj endemic area with high level Sj specific IgG4 antibodies. Then positive clones were preliminarily identified by PCR. Of the 8.6 × 104 phage plaques immunoscreeued by human specific IgG4 antibody, 11 are positive with the PCR products' size ranged from 564bp to 740bp.
Pages: 67-69
Year: 2000 Issue: 2
Journal: CHINESE JOURNAL OF ZOONOSES
Keyword: Schistosomajaponicum; Reinfected people; Immunoscreening; cDNA clones;
Abstract: To obtain waluable Schistosoma japonicum (Sj) antigen clones, an constructed Sj adult worm cDNA library was screened with serum from reinfected people of Sj endemic area with high level Sj specific IgG4 antibodies. Then positive clones were preliminarily identified by PCR. Of the 8.6 × 104 phage plaques immunoscreeued by human specific IgG4 antibody, 11 are positive with the PCR products' size ranged from 564bp to 740bp.
Related Articles