Construction of integrative vector for xylulokinase gene and its overexpression in Saccharomyces cerevisiae
Author(s): Jingping Ge, Xisheng Cao, Gang Song, Hongzhi Ling, Wenxiang Ping
Pages: 762-767
Year: 2010 Issue: 6
Journal: Acta Microbiologica Sinica
Abstract: [目的]以载体p406ADH1为构建骨架,构建一个酿酒酵母(Saccharomyces cerevisiae)工业菌株的整合表达载体.[方法]通过酶切连接的方式,将4个元件片段:作为筛选标记的G418抗性基因KanR,用于基因表达的ADH1终止子片段,酿酒酵母W5自身木酮糖激酶基因,18S rDNA介导的同源整合区,插入到骨架质粒p406ADH1中,得到多拷贝整合表达载体pCXS-RKTr.将该载...
Pages: 762-767
Year: 2010 Issue: 6
Journal: Acta Microbiologica Sinica
Abstract: [目的]以载体p406ADH1为构建骨架,构建一个酿酒酵母(Saccharomyces cerevisiae)工业菌株的整合表达载体.[方法]通过酶切连接的方式,将4个元件片段:作为筛选标记的G418抗性基因KanR,用于基因表达的ADH1终止子片段,酿酒酵母W5自身木酮糖激酶基因,18S rDNA介导的同源整合区,插入到骨架质粒p406ADH1中,得到多拷贝整合表达载体pCXS-RKTr.将该载...
Citations
- Development of stable flocculent Saccharomyees cerevisiae strain for continuous Aspergillus niger β-galactosidase production Carla O.José AT.Nelson L.Nancy ADS Lucilia D
- G418 selection and stability of cloned genes integrated at chromosomal δ sequences of Saccharomyces cerevisiae Wang X.Wang Z.Da Silva NA
- rDNA介导的多拷贝整合表达载体的构建及其在酿酒酵母工业菌株中的应用 刘向勇.沈煜.郭亭.鲍晓明
- Improvement in the anti-staling capability of beer by genetically modifying industrial brewing yeast with high glutathione content Jiang K.Li Q.Gu GX
- Towards industrial pentose-fermenting yeast strains Hahn-H(a)gerdal B.Karhumaa K.Fonseca C.SpencerMartins I Gorwa-Grauslund MF
- Ethanol production from acid hydrolysate of wood biomass using the flocculating yeast Saccharomyces cerevisiae strain KF-7 Tang YQ.An MZ.Liu K.Nagai S Shigematsu T Morimura S Kida K
- Changing flux of xylose metabolites by altering expression of xylose reductase and xylitol dehydrogenase in recombinant Saccharomyces cerevisiae Jin YS.Jeffries TW
- Genetic improvement of Saccharomyces cerevisiae for xylose fermentation Byron CH.Hung Lee
- Metabolic flux analysis of xylose metabolism in recombinant Saccharomyces cerevisiae using continuous euhure Pitkanen JP.Aristidou A.Salusj(a)rvi L.Ruohonen L Penttil(a) M
- Xylulokinase over expression in two strains of Saccharomyces cerevisiae also expressing xylose reduetase and xylitol dehydrogenase and its effect on fermentation of xylose and lignocellulosic hydrolysate Johansson B.Christensson C.Hobley T.HAHNH(A)GERDAL B
Related Articles