Screening and Identification of Amylase-producing Strain from Arctic Ocean and Optimization of Enzyme Producing Conditions
Author(s): CHEN Ji-gang, ZHANG Rong-rong, YANG Ji-fang, MAO Zhi-juan
Pages: 24-27,52
Year: 2010 Issue: 8
Journal: Agricultural Science & Technology
Keyword: Arctic sea; Amylase; Pseudoalteromonas;
Abstract: [目的]研究北冰洋产淀粉酶海洋细菌的筛选与鉴定及其产酶条件的优化.[方法]从156株北冰洋海洋细菌中筛选出淀粉酶高产菌株ArcB84A,并对其进行了菌株形态学鉴定、16S rRNA分子鉴定以及产酶条件优化.[结果]菌株ArcB84A属于交替假单胞菌属.菌株B84A的最佳产酶条件为:培养基起始pH值为7.0-8.0;最佳碳源为5‰葡萄糖;最佳氮源为5‰蛋白胨;TritonX-100、Tween20、...
Pages: 24-27,52
Year: 2010 Issue: 8
Journal: Agricultural Science & Technology
Keyword: Arctic sea; Amylase; Pseudoalteromonas;
Abstract: [目的]研究北冰洋产淀粉酶海洋细菌的筛选与鉴定及其产酶条件的优化.[方法]从156株北冰洋海洋细菌中筛选出淀粉酶高产菌株ArcB84A,并对其进行了菌株形态学鉴定、16S rRNA分子鉴定以及产酶条件优化.[结果]菌株ArcB84A属于交替假单胞菌属.菌株B84A的最佳产酶条件为:培养基起始pH值为7.0-8.0;最佳碳源为5‰葡萄糖;最佳氮源为5‰蛋白胨;TritonX-100、Tween20、...
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