Author(s): YUAN Li, HOU Dao-rong, XU Rong, XIA Biao, DE Wei, WANG Xin-ru
Pages: 2057-2060
Year: 2010 Issue:  11
Journal: Progress in Modern Biomedicine
Abstract: 目的:表达人卵泡刺激素受体(follicle-stimulating hormone receptor,FSHR)N端第18-34氨基酸片段.方法:将FSHR N端第18-34氨基酸片段克隆p-pGEX4T-1中,构建成重组质粒pGEX4T-1-FSHRN(18-34位氨基酸).将该重组质粒转化E.coli BL21后,IPTG诱导其表达,经亲和层析进行分离纯化,western blot鉴定.结果...