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Preliminary study on enzymatic extraction method and the effect of mung bean protein
Author(s): PAN Yan1, LV Chun-jian2, XIE Chuan-lei3, PEI Hai-run1, HE Cong-fen1, DONG Yin-mao1, WANG Chang-tao1, *
Pages: 238-
241
Year: 2010
Issue:
9
Journal: Science and Technology of Food Industry
Keyword: 绿豆蛋白; 高效液相色谱; 抗氧化;
Abstract: 利用酶法提取绿豆蛋白,并对其功效进行初步研究。单因素实验和正交优化实验结果表明,最佳酶解条件为加酶量7%、料液比为1:25、提取时间1h;对提取样品进行酪氨酸酶活性抑制实验、DPPH·清除实验和水杨酸法清除·OH实验,对其美白和抗氧化功效进行初步研究。结果显示,在样品浓度为10%时,酪氨酸酶抑制率为49·98%,DPPH·清除率为33·53%,·OH清除率为39·08%。利用高效液相色谱精确测定样品蛋白分子量分布,其分子量主要分布在408·41~1830·64之间。
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