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Study on determination of isomaltooligosaccharides in foods by high performance liquid chromatography method
Author(s): FU Bo-qiang1, WANG Jing1, TANG Zhi-yu1, WANG Yuan-xing2, XIE Ming-yong2, FU Fang-yuan1
Pages: 388-
391,395
Year: 2010
Issue:
10
Journal: Science and Technology of Food Industry
Keyword: 食品; 低聚异麦芽糖; 高效液相色谱法; 含量测定;
Abstract: 针对乳制品、固体饮料、糖果、保健品等食品中添加的功能活性成分低聚异麦芽糖,包括异麦芽糖(IG2)、潘糖(P)、异麦芽三糖(IG3)、异麦芽四糖(IG4)的检测,建立了水和乙腈为流动相,梯度洗脱,氨基色谱柱分离,蒸发光散射检测器(evaporative light-scattering detector,ELSD)检测食品中添加的低聚异麦芽糖含量的高效液相色谱方法。异麦芽糖、潘糖、异麦芽三糖、异麦芽四糖的线性工作范围分别为0.1~0.45mg/mL、0.1~0.45mg/mL、0.2~0.65mg/mL、0.1~0.35mg/mL,线性相关系数R2分别为1、0.9999、0.9998、0.9999。对不同类型的食品,四种低聚异麦芽糖的回收率在98.0%~105.0%之间,方法准确度高。室内重复性变异系数一般都低于10%;除了含量低的异麦芽四糖,室间再现性变异系数一般都低于10%,说明方法的重复性和再现性良好。该方法简单、易于掌握,测定结果准确,适于复杂食品基质中低聚异麦芽糖含量的测定。
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