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The application of minisatellite MS31A MVR- PCR digital coding technique in forensic science
Author(s): HOU Guangwei, JIANG Xianhua
Pages: 18-
20
Year: 2000
Issue:
1
Journal: JOURNAL OF FORENSIC MEDICINE
Keyword: 小卫星MS31A; MVR-PCR; 个体识别;
Abstract: 应用小卫星特异性引物31A、31A-A、31A-G与α-32PdCTP掺入扩增技术对MS31A(位于D7S21位点)进行MVR-PCR分析,成功地建立了一套适合于刑事案件中微量血痕、混合斑、毛发、骨组织等样品鉴定的快速、简便、准确的分析技术.灵敏度分析表明,该方法能检测出低至1ng的基因组DNA,具有较高的灵敏性.本技术在40余起强奸、凶杀等疑难案件中应用均获得成功.本研究还利用MVR数据库资料对实际案件中的犯罪嫌疑人成功地进行了筛选、排查以及串并案工作.
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