SERCA influences excitation-contraction coupling in heart failure rats
Author(s): WANG Yong-liang, FAN Xiao-yan, LIU Guan-sheng, HU Shu-ting, SHEN Ya-feng, TANG Ying, YANG Yong-ji
Pages: 486-490
Year: 2010 Issue: 5
Journal: Journal of Chinese Electron Microscopy Society
Abstract: 目的:研究钙泵介导的钙回摄对成年大鼠心室肌细胞兴奋收缩偶联功能的影响.方法:急性分离正常组和心衰组大鼠心室肌细胞,采用激光扫描共聚焦显微镜研究肌策网的钙容量,然后利用异丙肾上腺素(ISO,I μmol/L)激活心肌细胞β-肾上腺素受体后采用激光扫描共焦显微镜记录其对心肌细胞介导的钙释放;采用Western-blot分析心肌细胞肌浆网钙泵表达水平.结果:心衰组的钙容量显著低于对照组(n=10,P<0...
Pages: 486-490
Year: 2010 Issue: 5
Journal: Journal of Chinese Electron Microscopy Society
Abstract: 目的:研究钙泵介导的钙回摄对成年大鼠心室肌细胞兴奋收缩偶联功能的影响.方法:急性分离正常组和心衰组大鼠心室肌细胞,采用激光扫描共聚焦显微镜研究肌策网的钙容量,然后利用异丙肾上腺素(ISO,I μmol/L)激活心肌细胞β-肾上腺素受体后采用激光扫描共焦显微镜记录其对心肌细胞介导的钙释放;采用Western-blot分析心肌细胞肌浆网钙泵表达水平.结果:心衰组的钙容量显著低于对照组(n=10,P<0...
Citations
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- Desantiago J,Ai X,Islam M,et al.Arrhythmogenic effects of beta 2-adrenergic stimulation in the failing heart are attributable to enhanced sarcoplasmic reticulum Ca2+ load[J].Circ-Res,2008,102(11):1389-1397.
- Ding Y F,Brower G L,Zhong Q,et al.Defective intracellular Cat2+ homeostasis contributes to myocyte dysfunction during ventricular remodelling induced by chronic volume overload in rats[J].Clin-Exp-Pharmacol-Physiol,2008,35(7):827-835.
- Yamamoto T,Yano M.Identification of target domains of the cardiac ryanodine receptor to correct channel disorder in failing hearts[J].Circ,2008,117(3):762-768.
- Belevych A,Kubalova Z,Terentyev D,et al.Enhanced ryanodine receptor-mediated calcium leak determines reduced sarcoplasmic reticulum calcium content in chronic canine heart failure[J].Biophys J,2007,12,1,93(11):4083-4092.
- Izu L T,Means S A,Balke C W.Interplay of ryanodine receptor distribution and calcium dynamics[J].Biophys,2006,91(1):95-112.
- Cheng H,Lederer MR,Lederer WJ,et al.Calcium sparks and[Ca2+]i waves in cardiac myocytes[J].Cell Physiol,1996,270:148-159.
- Valeriy Lukyanenko,Inna Gyorke.Saisunder subramanian inhibition of Ca2+ sparks by ruthenium red in permeablilized rat ventricularmyocyres[J].Biophysical,2000,79:1273-1284.
- 曹春梅,张雄,陈莹莹.不同途径的心室肌细胞的分离.浙江大学学报(医学版)[J].2003,32(1):52-55.
- Gyorke S,Terentyev D.Modulation of ryanodine receptor by luminal calcium and accessory proteins in health and cardiac disease[J].Cardiovasc-Res,2008,77(2):245-255.
- Liu Y H,Yang X P,Nass O,et al.Chronic heart failure induced by coronary artery ligation in Lewis inbred rats[J].Am J Physiol Heart Circ Physio1,1997,272:H722-H727.
- Desantiago J,Ai X,Islam M,et al.Arrhythmogenic effects of beta 2-adrenergic stimulation in the failing heart are attributable to enhanced sarcoplasmic reticulum Ca2+ load[J].Circ-Res,2008,102(11):1389-1397.
- Ding Y F,Brower G L,Zhong Q,et al.Defective intracellular Cat2+ homeostasis contributes to myocyte dysfunction during ventricular remodelling induced by chronic volume overload in rats[J].Clin-Exp-Pharmacol-Physiol,2008,35(7):827-835.
- Yamamoto T,Yano M.Identification of target domains of the cardiac ryanodine receptor to correct channel disorder in failing hearts[J].Circ,2008,117(3):762-768.
- Belevych A,Kubalova Z,Terentyev D,et al.Enhanced ryanodine receptor-mediated calcium leak determines reduced sarcoplasmic reticulum calcium content in chronic canine heart failure[J].Biophys J,2007,12,1,93(11):4083-4092.
- Izu L T,Means S A,Balke C W.Interplay of ryanodine receptor distribution and calcium dynamics[J].Biophys,2006,91(1):95-112.
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