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Effect of transforming growth factor-β1 on thrombospondin-1 in hepatic fibrosis
Author(s): 
Pages: 1674-1676
Year: Issue:  11
Journal: Chinese Journal of Experimental Surgery

Keyword:  Hepatic fibrosisHepatic stellate cellsTransforming growth factor-β1;
Abstract: 目的 观察肝纤维化过程中转化生长因子(TGF)-β1对凝血酶敏感蛋白-1(TSP-1)合成的调节作用.方法 在从正常雄性Wistar大鼠获得的肝星形胶质细胞(HSC)中加入0、0.5、1.0、2.5、5.0 μg/L 5种浓度的TGF-β1,采用逆转录-聚合酶链反应法(RT-PCR)及实时荧光定量PCR(Real-time PCR)检测细胞中TSP-1 mRNA的表达及含量,酶联免疫吸附试验(EL...
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