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The effect of recombination adenovirus expressing nuclear factor-κBp65-specific small interference RNA in vivo
Pages: 1609-1611
Year: Issue:  11
Journal: Chinese Journal of Experimental Surgery

Keyword:  NF-κBsiRNAAdenovirus;
Abstract: 目的 在大鼠关节内观察核因子-κB(NF-κB)p65特异性小干涉核糖核酸(siRNA)重组腺病毒对靶基因的抑制作用.方法 在SD大鼠膝关节内,筛选合适的病毒剂量.利用β-gal染色鉴定重组腺病毒感染组织的特异性,应用凝胶电泳迁移率分析(EMSA)测定重组腺病毒对NF-κB转录活性的影响,应用Western blot测定重组腺病毒对软骨和滑膜细胞内NF-κB65的抑制作用.结果 重组腺病毒在大鼠膝...
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