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The development of ELISA-kit of quantitative analysis for penicillic acid from Penicillium cyclopium
Author(s): 
Pages: 14-16
Year: Issue:  8
Journal: Heilongjiang Animal Science and Veterinary Medicine

Abstract: 为了建立用于定量检测饲料与食品中青霉酸的ELISA试剂盒,试验在合成青霉酸人工抗原并免疫小鼠获得特异单克隆抗体的基础上,利用间接竞争ELISA方法对试剂盒各项技术参数进行测定,最终获得特异、快速的检测试剂盒.结果表明:在优化的条件下,该试剂盒对青霉酸标准品的最低检测浓度为1.6μg/mL;线性方程为y=9.54x+5.844 4(R<\'2>=0.996 4),线性范围为0.1~25.6μg/mL;...
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