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ji chuan ran xing zhi qi guan yan bing du ning xia zhu de fen li yu jian ding
Author(s): 
Pages: 117-119
Year: Issue:  8
Journal: Heilongjiang Animal Science and Veterinary Medicine

Abstract: 为了研究宁夏地区流行的鸡传染性支气管炎病毒生物学特性及流行疾病的特点,试验对分离株进行了病毒血凝特性试验、病毒干扰试验、鸡胚致病性试验、动物回归试验,随后采用RT-PCR技术对病毒的S1蛋白基因进行扩增和序列分析,并将病毒在传代Vero、BHK-21细胞系上盲传6代,同时提取细胞基因组RNA,采用RT-PGR扩增S1蛋白基因.结果表明:分离的病毒为鸡传染性支气管炎病毒,不能在传代细胞上进行适应培养...
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