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efs40 leng dong guan fa dui xiao shu pei tai leng dong xiao guo de tan suo
Author(s): 
Pages: 60-62
Year: Issue:  8
Journal: Heilongjiang Animal Science and Veterinary Medicine

Abstract:  自1972年Leibo S P[1]和Wimut I[2]开创性地冷冻保存哺乳动物胚胎试验成功以来,各国学者对胚胎冷冻方法进行了大量研究.Willadsen S M和Whittingham D G相继采用程序化慢速冷冻法冷冻保存小鼠未受精卵及早期胚胎获得成功;1985年,Rall W F等创建了玻璃化快速冷冻法;1987年Trounson A等利用塑料麦管冷冻法保存小鼠2-细胞期胚胎成功;199...
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