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zhu wen bing du rt-lamp kuai su jian ce fang fa de jian li he ying yong
Author(s): 
Pages: 121-123
Year: Issue:  8
Journal: Heilongjiang Animal Science and Veterinary Medicine

Abstract: 为了研究检测猪瘟病毒(CSFV)的快速诊断方法,试验采用反转录环介导等温扩增技术(RT-LAMP)分别设计了6条针对CSFV 5\'端保守基因特异引物,经条件优化后建立猪瘟病毒RT-LAMP快速检测方法.结果表明:在63℃恒温条件下45 min就能很好地扩增出CSFV目的片段;RT-LAMP产物经Xho Ⅰ酶切确定产物即为目的片段;将CSFV和其他6株与CSFV同属的病毒进行RT-LAMP扩增,结果...
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