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Enhancement of radiotherapy sensitivity of endometrial carcinoma cells by transfecting siRNA targeting Bcl2
Pages: 2688-2689
Year: Issue:  15
Journal: The Journal of Practical Medicine

Abstract: 目的:探讨bcl2-siRNA能否增加子宫内膜癌细胞放疗敏感性.方法:转染bcl2-siRNA入子宫内膜癌细胞株,6 h后照射(1、2、4、6 Gy),另设单纯照射组及空白对照.照射后24、48、72 h MTT法检测细胞生长抑制率.RT-PCR检测细胞bcl-2 mRNA表达水平,流式细胞仪检测细胞bcl-2蛋白表达率和细胞凋亡率.结果:转染bcl2-siRNA后,细胞bcl-2蛋白、mRNA表...
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