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A High Precision Algorithm for Fluorescence Single Molecule Localization with Nanometer Accuracy
Author(s): 
Pages: 394-400
Year: Issue:  5
Journal: Nanotechnology and Precision Engineering

Keyword:  荧光成像单分子纳米级定位图像信噪比二维高斯曲面;
Abstract: 荧光成像系统的技术指标、采集的荧光图像信噪比和单分子定位算法是影响单分子定位精度的3大因素.在介绍单分子定位的基本极限精度和理论计算精度的基础上,提出了一种被称为图像去噪高斯掩膜算法的单分子定位算法.依据实验用单分子成像系统的具体配置,通过计算机仿真表明,该算法良好的去噪处理和高斯加权质心运算方式,不仅能实现纳米级的定位精度,而且能突破一般理论计算精度的限制,接近基本极限精度,特别是在背景噪声较大...
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