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Comparision of Five Staining Methods on Mouse Round Spermatid
Author(s): 
Pages: 113-116
Year: Issue:  2
Journal: Journal of Biomedical Engineering Research

Keyword:  小鼠圆形精子鉴定染色方法;
Abstract: 比较五种染色方法对小鼠圆形精子(round spermatid,ROS)染色的效果,寻找一种鉴定圆形精子简单、有效的染色方法.以性成熟的昆明白鼠为实验材料,制备睾丸细胞悬液,分别用瑞氏、瑞氏-姬姆萨、姬姆萨、改良品红、苏木精五种方法对小鼠生精细胞进行染色,比较不同染色方法下圆形精子的形态.相比于其它四种染色方法,瑞氏-姬姆萨染色后能清晰地观察到圆形精子细胞膜和核膜的边缘,核质分明,与其它时期生精细...
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