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Sequencing and Analysis of the Intergenic Region of rRNA in Astragalus membranaceus Seeds
Author(s): 
Pages: 13162-13163
Year: Issue:  24
Journal: Journal of Anhui Agricultural Sciences

Abstract: [目的]通过对黄芪种子进行rRNA基因内转录间隔区碱基序列的测定,建立其分子水平的鉴定标记.[方法]以改良CTAB法提取黄芪种子DNA,利用合成的通用引物对其rRNA基因内转录间隔区进行nPCR扩增,将扩增产物进行碱基序列测定.[结果]琼脂糖凝胶电泳证实黄芪种子中rRNA基因内转录间隔区PCR扩增产物存在,经测序后得到了黄芪种子rRNA基因内转录间隔区的碱基序列.[结论]rRNA基因内转录间隔区碱...
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