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Production and Verification of Polyclonal Antibodies with Rocombinant LukF-PV
Author(s): 
Pages: 325-327
Year: Issue:  4
Journal: Journal of Clinical Transfusion and Laboratory Medicine

Abstract: 目的 制备重组金黄色葡萄球菌PV-杀白细胞素LukF-PV蛋白多克隆抗体并鉴定.方法 纯化获得重组蛋白LukF-PV,免疫新西兰白兔,收集多抗血清,ELISA法测定抗体滴度,Western blotting法鉴定免疫活性.结果 成功免疫新西兰白兔获得多抗血清,ELISA测定其效价为1∶103,Western blotting鉴定其能识别重组蛋白和金葡菌PVL蛋白.结论 成功制备出重组金黄色葡萄球菌...
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