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The Application of One-Step Real-Time Reverse Transcription PCR on Rapid Detection of Influenza Virus A
Author(s): 
Pages: 296-299
Year: Issue:  4
Journal: Journal of Clinical Transfusion and Laboratory Medicine

Abstract: 目的 建立一种能快速检测甲型流感病毒的一步法实时荧光PCR方法,对样本进行准确检测.方法 以甲型流感病毒特异M基因的保守区为靶区域设计特异引物和TaqMan荧光探针,建立一步法实时荧光RT-PCR方法,并对乙型流感病毒、呼吸道合胞病毒Long株、冠状病毒OC43及229E、副流感病毒1、2、3型以及季节性流感病毒H1、H3进行检测,验证该方法的特异性和灵敏度,并对临床样本进行检测应用.结果 所建立...
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