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The Optimization of SRAP Amplification Program and System in Non-heading Chinese Cabbage
Author(s): 
Pages: 119-122
Year: Issue:  4
Journal: Acta Agriculturae Boreali-Sinica

Keyword:  不结球白菜SRAPPCR正交试验;
Abstract: 以菜薹基因组DNA为模板,通过正交试验设计,从Mg2+、Taq酶、dNTPs、引物及模板5种因素4个水平对不结球白菜SRAP反应体系进行优化,建立了适合不结球白菜的SRAP-PCR优化反应体系.利用该优化体系,选用30对芸薹属SRAP引物,对5份不结球白菜的基因组进行扩增,筛选出7对具有2条以上特异扩增条带的SRAP引物,验证了该优化体系的稳定性.
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