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Establishment of C1R-AAD Cell Clone Stably Expressing HLA-A2 Restricted Multi-epitopes Gene of Hepatitis C Virus
Author(s): 
Pages: 683-686
Year: Issue:  7
Journal: Chinese Journal of Biologicals

Abstract: 目的 建立稳定表达丙型肝炎病毒(HCV)HLA-A2限制性多表位基因的C1R-AAD细胞克隆.方法 合成人泛素基因(Ub)和HCV HLA-A2限制性多表位基因(Mep),分别克隆入原核表达质粒pRSET-A,构建多表位抗原基因的原核表达质粒pRSET-Ub-Mep.用BamH Ⅰ和HindⅢ双酶切质粒pRSET-Ub-Mep,得到复合多表位基因Ub-Mep,亚克隆入真核表达载体pcD-NA3.1...
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