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Construction of Eukaryotic Expression Vector for Mouse MicroRNA miR-21 and Its Expression in Mouse Mesangial Cells
Author(s): 
Pages: 708-710
Year: Issue:  7
Journal: Chinese Journal of Biologicals

Abstract: 目的 构建小鼠微RNA miR-21真核表达质粒,并在小鼠肾小球系膜细胞中表达.方法 人工合成小鼠miR-21基因序列,构建miR-21真核表达质粒pGenesil-miR-21.使用脂质体Lipofectamine 2000转染小鼠肾小球系膜细胞后,G418筛选,获得稳定转染克隆,提取总RNA,通过实时荧光定量RT-PCR技术检测miR-21的表达.结果 经酶切鉴定和测序证实,合成的miR-21...
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