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Research on Cell Culture Characteristics of Hubei Strain of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus
Author(s): 
Pages: 103-106
Year: Issue:  9
Journal: Acta Agriculturae Jiangxi

Keyword:  高致病性猪蓝耳病病毒分离毒株增殖培养特性;
Abstract: 高致病性猪蓝耳病是近年来严重危害我国养猪业的一种传染病.为了探明高致病性猪蓝耳病病毒在Marc145细胞上的增殖特性,本研究在分离获得高致病性猪蓝耳病病毒湖北株的基础上进行了细胞培养特性研究.结果表明:高致病性猪蓝耳病病毒湖北株在Marc145细胞上增殖所需的最佳条件分别为:营养液最佳血清含量为10%;最佳pH值为7.2~7.4;细胞最佳培养密度为105~ 2×105/mL;病毒增殖的最佳接毒量为...
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