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Cloning and Expression of cDNA Fragment of Sucrose Phosphate Synthase in Fruit of Loquat
Author(s): 
Pages: 42-45
Year: Issue:  9
Journal: Acta Agriculturae Jiangxi

Keyword:  枇杷蔗糖磷酸合酶克隆表达分析;
Abstract: 根据GenBank中已登录的多种植物的蔗糖磷酸合成酶基因的保守序列设计引物,采用RT-PCR方法,从枇杷果实中扩增出该基因的cDNA片段并测序,分别为386 bp和383 bp.序列分析表明,枇杷果实蔗糖磷酸合成酶基因与桃果实蔗糖磷酸合成酶基因相似性达91%.半定量RT-PCR分析表明,该基因在枇杷果实生长发育过程中的表达量逐渐增加.所得基因片段已在GenBank中注册,386 bp和383 bp...
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