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Optimization of Extraction of Total DNA and ISSR-PCR System in Asparagus officinalis
Author(s): 
Pages: 38-41,45
Year: Issue:  9
Journal: Acta Agriculturae Jiangxi

Abstract: ISSR标记技术在芦笋上目前还未见相关的研究报道.本研究在改进芦笋基因组DNA提取方法基础上,以芦笋基因组总DNA为模板,对芦笋ISSR反应体系的重要参数进行优化试验,建立了一套适用于芦笋稳定可靠、重复性强的ISSR反应体系:25 μL的反应体系中,模板DNA量50 ng、Mg2+浓度2.0 mmol/L、正反引物各0.2 μmol/L、dNTPs 0.2 mmol/L、Taq DNA聚合酶1 U...
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