Author(s): LI Shiwang, CHEN Fen, OU Yangchi, CHEN Xiaozuo, MO Yanxiu, TANG Yinjuan, LIU Kefeng
Pages: 1637-1639
Year: 2010 Issue:  8
Journal: Chinese Journal of Pathophysiology
Keyword:  Caveolin-1; 寡核苷酸类; 反义; 细胞增殖; 血管内皮生长因子;
Abstract: 目的:研究caveolin-1反义寡核苷酸对肝癌细胞增殖和血管内皮生长因子(VEGF)表达的影响.方法:脂质体介导caveolin-1反义寡核苷酸瞬时转染SMMC7721细胞,Western blotting检测转染效果;MTF法和ELISA法分别检测转染前后SMMC7721细胞增殖和分泌VEGF的变化.结果:转染48 h后,反义寡核苷酸组caveolin-1蛋白表达水平明显低于对照组;MTT检测...