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Activation of polysaccharides from Polyporus umbellatus (Pers.)Fries on peritoneal macrophages from mice through Toll-like receptor 4
Author(s): XU Wen, LI Xinqun
Pages: 266-
273
Year: 2010
Issue:
4
Journal: Chinese Journal of Pharmacology and Toxicology
Abstract: 目的 观察猪苓多糖(PPS)对小鼠腹腔巨噬细胞的活化作用,并研究Toll样受体4(TLR4)在其中的作用.方法 用PES 12.5,25,50及100 mg·L-1刺激C3H/HeN小鼠和TLR4缺陷的C3H/HeJ小鼠脾细胞72 h或腹腔巨噬细胞24 h,以[3H]TdR掺入法检测脾细胞增殖反应;以Griess法测定腹腔巨噬细胞培养上清一氧化氮(NO)水平,ELISA检测白细胞介素1 β(IL-...
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