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Changes in expression level of heat shock protein 70 gene in Bemisia tabaci B-biotype (Homoptera: Aleyrodidae) under high temperature stress
Author(s): 
Pages: 1087-1091
Year: Issue:  11
Journal: ACTA ENTOMOLOGICA SINICA

Keyword:  B型烟粉虱高温胁迫热激蛋白基因hsp70表达量实时荧光定量RT-PCRTaqMan-MGB探针;
Abstract: 为了探讨热激蛋白在B型烟粉虱Bemisia tabaci B-biotype抵抗高温中的作用,以含有B型烟粉虱热激蛋白基因hsp70片段的质粒为模板,用TaqMan-MGB探针和特异性引物构建了B型烟粉虱hsp70实时荧光定量RT-PCR检测体系,并检测了37℃~45℃高温胁迫及气温变化时B型烟粉虱成虫体内hsp70表达的情况.结果表明:缓和的高温对B型烟粉虱成虫hsp70表达具有诱导作用,在37℃~41℃范围内,hsp70的表达量随着温度的升高从8.78×105±6.41×104拷贝数上升到1.99×107±1.45×105拷贝数,在41℃时达到最高峰;而当温度升高到43℃和45℃,hsp70表达量迅速降低.B型烟粉虱成虫hsp70的表达与昼夜气温变化有关,当上午气温从34℃上升到41℃时,hsp70的表达量从1.16×105±1.48×104拷贝数上升到6.29×106±1.80×105拷贝数;当傍晚气温下降到33℃时,hsp70表达量也下降到2.32×105±7.69×103拷贝数.因此推测,hsp70在B型烟粉虱抵抗高温胁迫方面可能具有重要作用.
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