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Construction and Analysis of Ovalbumin Secreted Vector for Human bFGF
Author(s): 
Pages: 328-331
Year: Issue:  3
Journal: Heilongjiang Medicine Journal

Keyword:  bFGF卵清蛋白分泌表达载体生物反应器;
Abstract: 目的:构建人碱性成纤维细胞生长因子(basic fibroblast growth factor, bFGF)的卵清蛋白分泌表达载体,为建立鸡输卵管生物反应器奠定基础.方法:利用PCR手段,从鸡基因组DNA克隆了4.1k(-4050bp~+42bp)卵清蛋白(ovalbumin)上游调控序列,利用重叠PCR技术,将溶菌酶信号肽(lysozyme signal peptide)基因与人碱性成纤维细胞...
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