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Determination of Calycosin and Formononetin in Radix Astragal by High Performance Liquid Chromatography
Author(s): 
Pages: 1541-1545
Year: Issue:  4
Journal: Chinese Journal of Spectroscopy Laboratory

Abstract: 利用化学计量学分辨方法和高效液相色谱法建立了黄芪中毛蕊异黄酮和芒柄花素的定量分析方法.色谱柱为Hypersic ODS柱(4.4mm×250mm,5μm),流动相为A:水含0.2%(V/V)醋酸,B:乙腈含0.2%(V/V)醋酸.线性梯度冼脱.借直观推导式演进特征投影(HELP)法分辨HPLC-DAD产生的二维数据得毛蕊异黄酮和芒柄花素的纯光谱和纯色谱.用总体积积分法定量分析其含量.毛蕊异黄酮和芒...
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