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A Study of Direct Competitive Immuno-Fluorescence for the Determination of Naphthalene in the Environment by Lumichrome Fluorescent Probe
Author(s): ZHUANG Huisheng, WANG Lili, ZHOU Chun
Pages: 28-
32
Year: 2010
Issue:
4
Journal: Environmental Monitoring in China
Keyword: 光色素; 萘; 荧光免疫分析; 荧光探针;
Abstract: 以光色素为荧光探针,将其标记到萘抗体上,采用直接荧光免疫分析的方法,对土壤环境中的萘(NA)进行了检测.优化了试验条件,选择洗涤液PBST的pH值为7.40,温育时间为2h,抗原浓度为40.0 μg/ml,标记抗体的稀释比为1∶ 20为最佳的实验条件.在此条件下,得到萘的标准工作曲线.线性范围是1.00×10-7~1.00×10-2 mg/ml,最低检出限为5.03×10-8mg/ml.并将这种方...
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