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Induction of prostate cancer-specific CTLs with dendritic cells pulsed by different types of tumor antigens
Pages: 410-414
Year: Issue:  5
Journal: National Journal of Andrology

Abstract: 目的:研究肿瘤冻融抗原及弱酸洗脱提取的肿瘤抗原肽分别冲击致敏树突状细胞(dendritic cells,DCs)体外诱导前列腺癌特异性细胞毒性T淋巴细胞(CTL)的效应. 方法:采用反复冻融法和弱酸洗脱法,分别获取前列腺癌细胞株PC-3的肿瘤抗原,应用重组人GM-CSF、IL4体外培养诱导外周血单个核细胞获得DC,并分别负载两种前列腺癌肿瘤抗原,检测其体外激活肿瘤特异性CTL的能力,用LDH释放法...
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