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Modified serum-guided immunoblotting for differential proteomic study of prostate cancer
Author(s): 
Pages: 438-444
Year: Issue:  5
Journal: National Journal of Andrology

Keyword:  雄激素依赖型前列腺癌激素难治性前列腺癌蛋白质组学免疫印迹;
Abstract: 目的:探讨新的方法筛选发现前列腺癌的分子干预靶点. 方法:收集2007年2月至2008年4月间我院收治手术切除前列腺癌组织和良性前列腺增生组织及其配对的患者血清标本3例.提取组织总蛋白,双向电泳分离并转膜,正常人血清封闭.患者血清作为一抗杂交,行Western印迹检测,前后比较差异表达蛋白,MALDITOF-MS质谱分析,数据库查询鉴定后进行RT-PCR法和Western印迹验证. 结果:该方法改...
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